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Microbiology

Critical Thinking

MicrobiologyCritical Thinking

Critical Thinking

32.

Is biotechnology always associated with genetic engineering? Explain your answer.

33.

Which is more efficient: blunt-end cloning or sticky-end cloning? Why?

34.

Suppose you are working in a molecular biology laboratory and are having difficulty performing the PCR successfully. You decide to double-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effects would this mistake have on the PCR reaction? Refer to Figure 12.20.

35.

What is the advantage of microarray analysis over northern blot analysis in monitoring changes in gene expression?

36.

What is the difference between reverse transcriptase PCR (RT-PCR) and real-time quantitative PCR (qPCR)?

37.

What are some advantages of cloning human genes into bacteria to treat human diseases caused by specific protein deficiencies?

38.

Compare the ethical issues involved in the use of somatic cell gene therapy and germ-line gene therapy.

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