Skip to ContentGo to accessibility pageKeyboard shortcuts menu
OpenStax Logo

Critical Thinking

MicrobiologyCritical Thinking

Critical Thinking


Is biotechnology always associated with genetic engineering? Explain your answer.


Which is more efficient: blunt-end cloning or sticky-end cloning? Why?


Suppose you are working in a molecular biology laboratory and are having difficulty performing the PCR successfully. You decide to double-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effects would this mistake have on the PCR reaction? Refer to Figure 12.20.


What is the advantage of microarray analysis over northern blot analysis in monitoring changes in gene expression?


What is the difference between reverse transcriptase PCR (RT-PCR) and real-time quantitative PCR (qPCR)?


What are some advantages of cloning human genes into bacteria to treat human diseases caused by specific protein deficiencies?


Compare the ethical issues involved in the use of somatic cell gene therapy and germ-line gene therapy.

Order a print copy

As an Amazon Associate we earn from qualifying purchases.


This book may not be used in the training of large language models or otherwise be ingested into large language models or generative AI offerings without OpenStax's permission.

Want to cite, share, or modify this book? This book uses the Creative Commons Attribution License and you must attribute OpenStax.

Attribution information
  • If you are redistributing all or part of this book in a print format, then you must include on every physical page the following attribution:
    Access for free at
  • If you are redistributing all or part of this book in a digital format, then you must include on every digital page view the following attribution:
    Access for free at
Citation information

© Jan 10, 2024 OpenStax. Textbook content produced by OpenStax is licensed under a Creative Commons Attribution License . The OpenStax name, OpenStax logo, OpenStax book covers, OpenStax CNX name, and OpenStax CNX logo are not subject to the Creative Commons license and may not be reproduced without the prior and express written consent of Rice University.