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Microbiology

Short Answer

MicrobiologyShort Answer

Short Answer

49.

Can two observably different cells have the same genotype? Explain.

50.

Why is primase required for DNA replication?

51.

What is the role of single-stranded binding protein in DNA replication?

52.

Below is a DNA sequence. Envision that this is a section of a DNA molecule that has separated in preparation for replication, so you are only seeing one DNA strand. Construct the complementary DNA sequence (indicating 5’ and 3’ ends).

DNA sequence: 3’-T A C T G A C T G A C G A T C-5’

53.

What is the purpose of RNA processing in eukaryotes? Why don’t prokaryotes require similar processing?

54.

Below is a DNA sequence. Envision that this is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the antisense strand. Construct the mRNA sequence transcribed from this template.

Antisense DNA strand: 3’-T A C T G A C T G A C G A T C-5’

55.

Why does translation terminate when the ribosome reaches a stop codon? What happens?

56.

How does the process of translation differ between prokaryotes and eukaryotes?

57.

What is meant by the genetic code being nearly universal?

58.

Below is an antisense DNA sequence. Translate the mRNA molecule synthesized using the genetic code, recording the resulting amino acid sequence, indicating the N and C termini.

Antisense DNA strand: 3’-T A C T G A C T G A C G A T C-5’

59.

Why is it more likely that insertions or deletions will be more detrimental to a cell than point mutations?

60.

Briefly describe two ways in which chromosomal DNA from a donor cell may be transferred to a recipient cell during the process of conjugation.

61.

Describe what happens when a nonsense mutation is introduced into the gene encoding transposase within a transposon.

62.

What are two ways that bacteria can influence the transcription of multiple different operons simultaneously in response to a particular environmental condition?

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