By the end of this section, you will be able to:
- Describe how a water molecule is polar.
- Explain electrostatic screening by a water molecule within a living cell.
Classical electrostatics has an important role to play in modern molecular biology. Large molecules such as proteins, nucleic acids, and so on—so important to life—are usually electrically charged. DNA itself is highly charged; it is the electrostatic force that not only holds the molecule together but gives the molecule structure and strength. Figure 18.32 is a schematic of the DNA double helix.
The four nucleotide bases are given the symbols A (adenine), C (cytosine), G (guanine), and T (thymine). The order of the four bases varies in each strand, but the pairing between bases is always the same. C and G are always paired and A and T are always paired, which helps to preserve the order of bases in cell division (mitosis) so as to pass on the correct genetic information. Since the Coulomb force drops with distance (), the distances between the base pairs must be small enough that the electrostatic force is sufficient to hold them together.
DNA is a highly charged molecule, with about (fundamental charge) per m. The distance separating the two strands that make up the DNA structure is about 1 nm, while the distance separating the individual atoms within each base is about 0.3 nm.
One might wonder why electrostatic forces do not play a larger role in biology than they do if we have so many charged molecules. The reason is that the electrostatic force is “diluted” due to screening between molecules. This is due to the presence of other charges in the cell.
Polarity of Water Molecules
The best example of this charge screening is the water molecule, represented as . Water is a strongly polar molecule. Its 10 electrons (8 from the oxygen atom and 2 from the two hydrogen atoms) tend to remain closer to the oxygen nucleus than the hydrogen nuclei. This creates two centers of equal and opposite charges—what is called a dipole, as illustrated in Figure 18.33. The magnitude of the dipole is called the dipole moment.
These two centers of charge will terminate some of the electric field lines coming from a free charge, as on a DNA molecule. This results in a reduction in the strength of the Coulomb interaction. One might say that screening makes the Coulomb force a short range force rather than long range.
Other ions of importance in biology that can reduce or screen Coulomb interactions are and and . These ions are located both inside and outside of living cells. The movement of these ions through cell membranes is crucial to the motion of nerve impulses through nerve axons.
Recent studies of electrostatics in biology seem to show that electric fields in cells can be extended over larger distances, in spite of screening, by “microtubules” within the cell. These microtubules are hollow tubes composed of proteins that guide the movement of chromosomes when cells divide, the motion of other organisms within the cell, and provide mechanisms for motion of some cells (as motors).
You are likely familiar with the role of electrical signals in nerve conduction and the importance of charges in cardiac and related activity. Changes in electrical properties are also essential in core biological processes. Ernest Everett Just, whose expertise in understanding and handling egg cells led to a number of critical experimental discoveries, investigated the role of the cell membrane in reproductive fertilization. In one key experiment, Just established that the egg membrane undergoes a depolarizing "wave of negativity" the moment it fuses with a sperm cell. This change in charge is now known as the "fast block" that ensures that only one sperm cell fuses with an egg cell and is critical for embryonic development.
Bioelectricity and Wound Healing
Just as electrical forces drive activities in healthy cells and systems, they are also critical in damaged ones. Scientists have long known that injuries or infections are managed by the body through various responses, including increased white blood cell concentrations, swelling, and tissue repair. For example, human cells damaged by wounds heal through a complex process. But what triggers it?
Physicists and biologists working together at Vanderbilt University used an ultra-precise laser to uncover the processes organisms use to repair damage. Lead researchers Andrea Page-Degraw and Shane Hutson and study author Erica Shannon discovered that immediately upon damage, cells release calcium ions and eventually other molecules, driving an electrochemical response that initiates the healing process. Shannon notes that different types of damage lead to different chemical releases, demonstrating how organisms may initiate specific responses to best address the injury.
While far more research is required to understand the triggering and response method, other research indicates that bioelectricity is highly involved in wound healing. Several studies have indicated that precise and low-level electrical stimulation of wounds (such as those from surgeries) leads to faster healing. While the mechanisms are not fully understood, electrical stimulation is a growing area of research and practice in medicine.