Learning Objectives
By the end of this section, you will be able to:
- Identify and describe the properties of life. Why and how does passive transport occur across membranes?
- What is tonicity, and how is it relevant to passive transport?
Connection for AP® Courses
Preventing dehydration is important for both plants and animals. Water moves across plasma membranes by a specific type of diffusion called osmosis. The concentration gradient of water across a membrane is inversely proportional to the concentration of solutes; that is, water moves through channel proteins called aquaporins from higher water concentration to lower water concentration. Solute concentration outside and inside the cell influences the rate of osmosis. Tonicity describes how the extracellular concentration of solutes can change the volume of a cell by affecting osmosis, often correlating with the osmolarity of the solution, i.e., the total solute concentration of the solution. In a hypotonic situation, because the extracellular fluid has a lower concentration of solutes (lower osmolarity) than the fluid inside the cell, water enters the cell, causing it to swell and possibly burst. The cell walls of plants prevent them from bursting, but animal cells, such as red blood cells, can lyse. When a cell is placed in a hypertonic solution, water leaves the cell because the cell has a higher water potential than the extracellular solution. When the concentrations of solute are equal on both sides of the membrane (isotonic), no net movement of water into or out of the cell occurs. Living organisms have evolved a variety of ways to maintain osmotic balance; for example, marine fish secrete excess salt through the gills to maintain dynamic homeostasis.
Information presented and the examples highlighted in the section support concepts and learning objectives outlined in Big Idea 2 of the AP® Biology Curriculum Framework. The learning objectives listed in the Curriculum Framework provide a transparent foundation for the AP® Biology course, an inquiry-based laboratory experience, instructional activities, and AP® exam questions. A learning objective merges required content with one or more of the seven science practices.
| Essential Knowledge | 2.B.2 Growth and dynamic homeostasis are maintained by the constant movement of molecules across membranes. |
| Science Practice | 1.4 The student can use representations and models to analyze situations or solve problems qualitatively and quantitatively. |
| Science Practice | 3.1 The student can pose scientific questions. |
| Learning Objective | 2.11 The student is able to construct models that connect the movement of molecules across membranes with membrane structure and function. |
| Essential Knowledge | 2.B.2 Growth and dynamic homeostasis are maintained by the constant movement of molecules across membranes. |
| Science Practice | 1.4 The student can use representations and models to analyze situations or solve problems qualitatively and quantitatively. |
| Science Practice | 3.1 The student can pose scientific questions. |
| Learning Objective | 2.12 The student is able to use representations and models to analyze situation or solve problems qualitatively and quantitatively to investigate whether dynamic homeostasis is maintained by the active movement of molecules across membranes. |
Teacher Support
Students may think that diffusion and osmosis are identical and the terms are interchangeable. Discuss with students the difference between diffusion and osmosis. Diffusion is the movement of solutes from an area of high concentration to an area of lower concentration. Osmosis is the movement of free water molecules through a semipermeable membrane according to the water's concentration gradient across the membrane, which is inversely proportional to the solutes' concentration. In diffusion, the solutes move. In osmosis, the water moves. In both, the goal is the same: to balance out the solute concentration.
The Science Practice Challenge Questions contain additional test questions for this section that will help you prepare for the AP exam. These questions address the following standards:
[APLO 2.25][APLO 2.27][APLO 4.3][APLO 4.17][APLO1.9] [APLO 2.16][APLO 2.17][APLO 2.18]
Plasma membranes must allow certain substances to enter and leave a cell, and prevent some harmful materials from entering and some essential materials from leaving. In other words, plasma membranes are selectively permeable— (semipermeable)they allow some substances to pass through, but not others. If they were to lose this selectivity, the cell would no longer be able to sustain itself, and it would be destroyed. Some cells require larger amounts of specific substances than do other cells; they must have a way of obtaining these materials from extracellular fluids. This may happen passively, as certain materials move back and forth, or the cell may have special mechanisms that facilitate transport. Some materials are so important to a cell that it spends some of its energy, hydrolyzing adenosine triphosphate (ATP), to obtain these materials. Red blood cells use some of their energy doing just that. Most cells spend the majority of their energy to maintain an imbalance of sodium and potassium ions between the interior and exterior of the cell.
The most direct forms of membrane transport are passive. Passive transport is a naturally occurring phenomenon and does not require the cell to exert any of its energy to accomplish the movement. In passive transport, substances move from an area of higher concentration to an area of lower concentration. A physical space in which there is a range of concentrations of a single substance is said to have a concentration gradient.
Selective Permeability
Plasma membranes are asymmetric: the interior of the membrane is not identical to the exterior of the membrane. In fact, there is a considerable difference between the array of phospholipids and proteins between the two leaflets that form a membrane. On the interior of the membrane, some proteins serve to anchor the membrane to fibers of the cytoskeleton. There are peripheral proteins on the exterior of the membrane that bind elements of the extracellular matrix. Carbohydrates, attached to lipids or proteins, are also found on the exterior surface of the plasma membrane. These carbohydrate complexes help the cell bind substances that the cell needs in the extracellular fluid. This adds considerably to the selective nature of plasma membranes (Figure 5.7).
Recall that plasma membranes are amphiphilic: They have hydrophilic and hydrophobic regions. This characteristic helps the movement of some materials through the membrane and hinders the movement of others. Lipid-soluble material with a low molecular weight can easily slip through the hydrophobic lipid core of the membrane. Substances such as the fat-soluble vitamins A, D, E, and K readily pass through the plasma membranes in the digestive tract and other tissues. Fat-soluble drugs and hormones also gain easy entry into cells and are readily transported into the body’s tissues and organs. Similarly, molecules of oxygen and carbon dioxide have no charge and so pass through membranes by simple diffusion.
Polar substances present problems for the membrane. While some polar molecules connect easily with the outside of a cell, they cannot readily pass through the lipid core of the plasma membrane. Additionally, while small ions could easily slip through the spaces in the mosaic of the membrane, their charge prevents them from doing so. Ions such as sodium, potassium, calcium, and chloride must have special means of penetrating plasma membranes. Simple sugars and amino acids also need help with transport across plasma membranes, achieved by various transmembrane proteins (channels).
Diffusion
Diffusion is a passive process of transport. A single substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across a space. You are familiar with diffusion of substances through the air. For example, think about someone opening a bottle of ammonia in a room filled with people. The ammonia gas is at its highest concentration in the bottle; its lowest concentration is at the edges of the room. The ammonia vapor will diffuse, or spread away, from the bottle, and gradually, more and more people will smell the ammonia as it spreads. Materials move within the cell’s cytosol by diffusion, and certain materials move through the plasma membrane by diffusion (Figure 5.8). Diffusion expends no energy. On the contrary, concentration gradients are a form of potential energy, dissipated as the gradient is eliminated.
Each separate substance in a medium, such as the extracellular fluid, has its own concentration gradient, independent of the concentration gradients of other materials. In addition, each substance will diffuse according to that gradient. Within a system, there will be different rates of diffusion of the different substances in the medium.
Factors That Affect Diffusion
Molecules move constantly in a random manner, at a rate that depends on their mass, their environment, and the amount of thermal energy they possess, which in turn is a function of temperature. This movement accounts for the diffusion of molecules through whatever medium in which they are localized. A substance will tend to move into any space available to it until it is evenly distributed throughout it. After a substance has diffused completely through a space, removing its concentration gradient, molecules will still move around in the space, but there will be no net movement of the number of molecules from one area to another. This lack of a concentration gradient in which there is no net movement of a substance is known as dynamic equilibrium. While diffusion will go forward in the presence of a concentration gradient of a substance, several factors affect the rate of diffusion.
- Extent of the concentration gradient: The greater the difference in concentration, the more rapid the diffusion. The closer the distribution of the material gets to equilibrium, the slower the rate of diffusion becomes.
- Mass of the molecules diffusing: Heavier molecules move more slowly; therefore, they diffuse more slowly. The reverse is true for lighter molecules.
- Temperature: Higher temperatures increase the energy and therefore the movement of the molecules, increasing the rate of diffusion. Lower temperatures decrease the energy of the molecules, thus decreasing the rate of diffusion.
- Solvent density: As the density of a solvent increases, the rate of diffusion decreases. The molecules slow down because they have a more difficult time getting through the denser medium. If the medium is less dense, diffusion increases. Because cells primarily use diffusion to move materials within the cytoplasm, any increase in the cytoplasm’s density will inhibit the movement of the materials. An example of this is a person experiencing dehydration. As the body’s cells lose water, the rate of diffusion decreases in the cytoplasm, and the cells’ functions deteriorate. Neurons tend to be very sensitive to this effect. Dehydration frequently leads to unconsciousness and possibly coma because of the decrease in diffusion rate within the cells.
- Solubility: As discussed earlier, nonpolar or lipid-soluble materials pass through plasma membranes more easily than polar materials, allowing a faster rate of diffusion.
- Surface area and thickness of the plasma membrane: Increased surface area increases the rate of diffusion, whereas a thicker membrane reduces it.
- Distance travelled: The greater the distance that a substance must travel, the slower the rate of diffusion. This places an upper limitation on cell size. A large, spherical cell will die because nutrients or waste cannot reach or leave the center of the cell, respectively. Therefore, cells must either be small in size, as in the case of many prokaryotes, or be flattened, as with many single-celled eukaryotes.
A variation of diffusion is the process of filtration. In filtration, material moves according to its concentration gradient through a membrane; sometimes the rate of diffusion is enhanced by pressure, causing the substances to filter more rapidly. This occurs in the kidney, where blood pressure forces large amounts of water and accompanying dissolved substances, or solutes, out of the blood and into the renal tubules. The rate of diffusion in this instance is almost totally dependent on pressure. One of the effects of high blood pressure is the appearance of protein in the urine, which is “squeezed through” by the abnormally high pressure.
Facilitated transport
In facilitated transport, also called facilitated diffusion, materials diffuse across the plasma membrane with the help of membrane proteins. A concentration gradient exists that would allow these materials to diffuse into the cell without expending cellular energy. However, these materials are polar molecules that are repelled by the hydrophobic parts of the cell membrane. Facilitated transport proteins shield these materials from the repulsive force of the membrane, allowing them to diffuse into the cell.
The material being transported is first attached to protein or glycoprotein receptors on the exterior surface of the plasma membrane. This allows the material that is needed by the cell to be removed from the extracellular fluid. The substances are then passed to specific integral proteins that facilitate their passage. Some of these integral proteins are collections of beta pleated sheets that form a pore or channel through the phospholipid bilayer. Others are carrier proteins which bind with the substance and aid its diffusion through the membrane.
Channels
The integral proteins involved in facilitated transport are collectively referred to as transport proteins, and they function as either channels for the material or carriers. In both cases, they are transmembrane proteins. Channels are specific for the substance that is being transported. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids; they additionally have a hydrophilic channel through their core that provides a hydrated opening through the membrane layers (Figure 5.9). Passage through the channel allows polar compounds to avoid the nonpolar central layer of the plasma membrane that would otherwise slow or prevent their entry into the cell. Aquaporins are channel proteins that allow water to pass through the membrane at a very high rate.
Channel proteins are either open at all times or they are “gated,” which controls the opening of the channel. The attachment of a particular ion to the channel protein may control the opening, or other mechanisms or substances may be involved. In some tissues, sodium and chloride ions pass freely through open channels, whereas in other tissues a gate must be opened to allow passage. An example of this occurs in the kidney, where both forms of channels are found in different parts of the renal tubules. Cells involved in the transmission of electrical impulses, such as nerve and muscle cells, have gated channels for sodium, potassium, and calcium in their membranes. Opening and closing of these channels changes the relative concentrations on opposing sides of the membrane of these ions, resulting in the facilitation of electrical transmission along membranes (in the case of nerve cells) or in muscle contraction (in the case of muscle cells).
Carrier Proteins
Another type of protein embedded in the plasma membrane is a carrier protein. This aptly named protein binds a substance and, in doing so, triggers a change of its own shape, moving the bound molecule from the outside of the cell to its interior (Figure 5.10); depending on the gradient, the material may move in the opposite direction. Carrier proteins are typically specific for a single substance. This selectivity adds to the overall selectivity of the plasma membrane. The exact mechanism for the change of shape is poorly understood. Proteins can change shape when their hydrogen bonds are affected, but this may not fully explain this mechanism. Each carrier protein is specific to one substance, and there are a finite number of these proteins in any membrane. This can cause problems in transporting enough of the material for the cell to function properly. When all of the proteins are bound to their ligands, they are saturated and the rate of transport is at its maximum. Increasing the concentration gradient at this point will not result in an increased rate of transport.
An example of this process occurs in the kidney. Glucose, water, salts, ions, and amino acids needed by the body are filtered in one part of the kidney. This filtrate, which includes glucose, is then reabsorbed in another part of the kidney. Because there are only a finite number of carrier proteins for glucose, if more glucose is present than the proteins can handle, the excess is not transported and it is excreted from the body in the urine. In a diabetic individual, this is described as “spilling glucose into the urine.” A different group of carrier proteins called glucose transport proteins, or GLUTs, are involved in transporting glucose and other hexose sugars through plasma membranes within the body.
Channel and carrier proteins transport material at different rates. Channel proteins transport much more quickly than do carrier proteins. Channel proteins facilitate diffusion at a rate of tens of millions of molecules per second, whereas carrier proteins work at a rate of a thousand to a million molecules per second.
Osmosis
Osmosis is the movement of free water molecules through a semipermeable membrane according to the water's concentration gradient across the membrane, which is inversely proportional to the solutes' concentration. While diffusion transports material across membranes and within cells, osmosis transports only water across a membrane and the membrane limits the diffusion of solutes in the water. Not surprisingly, the aquaporins that facilitate water movement play a large role in osmosis, most prominently in red blood cells and the membranes of kidney tubules.
Mechanism
Osmosis is a special case of diffusion. Water, like other substances, moves from an area of high concentration of free water molecules to one of low free water molecule concentration. An obvious question is what makes water move at all? Imagine a beaker with a semipermeable membrane separating the two sides or halves (Figure 5.11). On both sides of the membrane the water level is the same, but there are different concentrations of a dissolved substance, or solute, that cannot cross the membrane (otherwise the concentrations on each side would be balanced by the solute crossing the membrane). If the volume of the solution on both sides of the membrane is the same, but the concentrations of solute are different, then there are different amounts of water, the solvent, on either side of the membrane.
To illustrate this, imagine two full glasses of water. One has a single teaspoon of sugar in it, whereas the second one contains one-quarter cup of sugar. If the total volume of the solutions in both cups is the same, which cup contains more water? Because the large amount of sugar in the second cup takes up much more space than the teaspoon of sugar in the first cup, the first cup has more water in it.
Returning to the beaker example, recall that it has a mixture of solutes on either side of the membrane. A principle of diffusion is that the molecules move around and will spread evenly throughout the medium if they can. However, only the material capable of getting through the membrane will diffuse through it. In this example, the solute cannot diffuse through the membrane, but the water can. Water has a concentration gradient in this system. Thus, water will diffuse down its concentration gradient, crossing the membrane to the side where it is less concentrated. This diffusion of water through the membrane—osmosis—will continue until the concentration gradient of water goes to zero or until the hydrostatic pressure of the water balances the osmotic pressure. Osmosis proceeds constantly in living systems.
The beaker example here occurs in an open system where the volume of fluid can increase and decrease freely. Cells, on the other hand, are composed of proteins and other substances embedded in the aqueous cytoplasm. These substances could be considered solutes for the purposes of predicting osmosis. The cell membrane keeps most of the proteins and other substances within the cell, causing the cell to have a higher osmolarity than pure water.
Suppose you perform an experiment where you placed red blood cells in an environment of pure water. What do you suppose would happen to the cells? Because the concentration of solute is higher in the red blood cell than it is in the beaker, water would rush into the red blood cell. What do you think would happen to the red blood cell, given that its cell membrane is made up of a fixed surface area? It is likely that the red blood cell will undergo hemolysis, where they swell up with water and burst. It should be noted, however, that most cells have mechanisms to prevent them from taking on too much water. However, red blood cells lack these controls, making them ideal for osmolarity studies.
This is an important consideration for clinicians delivering drugs intravenously. How would the drug have to be formulated, in terms of osmolarity, to prevent red blood cells from undergoing hemolysis? In order to prevent hemolysis of red blood cells in the blood, drugs are typically formulated in an isotonic solution with the blood to maintain osmolarity.
Tonicity
Tonicity describes how an extracellular solution can change the volume of a cell by affecting osmosis. A solution's tonicity often directly correlates with the osmolarity of the solution. Osmolarity describes the total solute concentration of the solution. A solution with low osmolarity has a greater number of water molecules relative to the number of solute particles; a solution with high osmolarity has fewer water molecules with respect to solute particles. In a situation in which solutions of two different osmolarities are separated by a membrane permeable to water, though not to the solute, water will move from the side of the membrane with lower osmolarity (and more water) to the side with higher osmolarity (and less water). This effect makes sense if you remember that the solute cannot move across the membrane, and thus the only component in the system that can move—the water—moves along its own concentration gradient. An important distinction that concerns living systems is that osmolarity measures the number of particles (which may be molecules) in a solution. Therefore, a solution that is cloudy with cells may have a lower osmolarity than a solution that is clear, if the second solution contains more dissolved molecules than there are cells.
Hypotonic Solutions
Three terms—hypotonic, isotonic, and hypertonic—are used to relate the osmolarity of a cell to the osmolarity of the extracellular fluid that contains the cells. In a hypotonic situation, the extracellular fluid has lower osmolarity than the fluid inside the cell, and water enters the cell. (In living systems, the point of reference is always the cytoplasm, so the prefix hypo- means that the extracellular fluid has a lower concentration of solutes, or a lower osmolarity, than the cell cytoplasm.) It also means that the extracellular fluid has a higher concentration of water in the solution than does the cell. In this situation, water will follow its concentration gradient and enter the cell.
Hypertonic Solutions
As for a hypertonic solution, the prefix hyper- refers to the extracellular fluid having a higher osmolarity than the cell’s cytoplasm; therefore, the fluid contains less water than the cell does. Because the cell has a relatively higher concentration of water, water will leave the cell.
Isotonic Solutions
In an isotonic solution, the extracellular fluid has the same osmolarity as the cell. If the osmolarity of the cell matches that of the extracellular fluid, there will be no net movement of water into or out of the cell, although water will still move in and out. Blood cells and plant cells in hypertonic, isotonic, and hypotonic solutions take on characteristic appearances (Figure 5.12).
Link to Learning
For a video illustrating the process of diffusion in solutions, visit this site.
Tonicity in Living Systems
In a hypotonic environment, water enters a cell, and the cell swells. In an isotonic condition, the relative concentrations of solute and solvent are equal on both sides of the membrane. There is no net water movement; therefore, there is no change in the size of the cell. In a hypertonic solution, water leaves a cell and the cell shrinks. If either the hypo- or hyper- condition goes to excess, the cell’s functions become compromised, and the cell may be destroyed.
A red blood cell will burst, or lyse, when it swells beyond the plasma membrane’s capability to expand. Remember, the membrane resembles a mosaic, with discrete spaces between the molecules composing it. If the cell swells, and the spaces between the lipids and proteins become too large, the cell will break apart.
In contrast, when excessive amounts of water leave a red blood cell, the cell shrinks, or crenates. This has the effect of concentrating the solutes left in the cell, making the cytosol denser and interfering with diffusion within the cell. The cell’s ability to function will be compromised and may also result in the death of the cell.
Various living things have ways of controlling the effects of osmosis—a mechanism called osmoregulation. Some organisms, such as plants, fungi, bacteria, and some protists, have cell walls that surround the plasma membrane and prevent cell lysis in a hypotonic solution. The plasma membrane can only expand to the limit of the cell wall, so the cell will not lyse. In fact, the cytoplasm in plants is always slightly hypertonic to the cellular environment, and water will always enter a cell if water is available. This inflow of water produces turgor pressure, which stiffens the cell walls of the plant (Figure 5.13). In nonwoody plants, turgor pressure supports the plant. Conversly, if the plant is not watered, the extracellular fluid will become hypertonic, causing water to leave the cell. In this condition, the cell does not shrink because the cell wall is not flexible. However, the cell membrane detaches from the wall and constricts the cytoplasm. This is called plasmolysis. Plants lose turgor pressure in this condition and wilt (Figure 5.14).
Tonicity is a concern for all living things. For example, paramecia and amoebas, which are protists that lack cell walls, have contractile vacuoles. This vesicle collects excess water from the cell and pumps it out, keeping the cell from lysing as it takes on water from its environment (Figure 5.15).
Many marine invertebrates have internal salt levels matched to their environments, making them isotonic with the water in which they live. Fish, however, must spend approximately five percent of their metabolic energy maintaining osmotic homeostasis. Freshwater fish live in an environment that is hypotonic to their cells. These fish actively take in salt through their gills and excrete diluted urine to rid themselves of excess water. Saltwater fish live in the reverse environment, which is hypertonic to their cells, and they secrete salt through their gills and excrete highly concentrated urine.
In vertebrates, the kidneys regulate the amount of water in the body. Osmoreceptors are specialized cells in the brain that monitor the concentration of solutes in the blood. If the levels of solutes increase beyond a certain range, a hormone is released that retards water loss through the kidney and dilutes the blood to safer levels. Animals also have high concentrations of albumin, which is produced by the liver, in their blood. This protein is too large to pass easily through plasma membranes and is a major factor in controlling the osmotic pressures applied to tissues.
Science Practice Connection for AP® Courses
Activity
Use the model of the plasma cell membrane you constructed to demonstrate how O2 and CO2, H2O, Na+ and K+, and glucose are transported across the membrane.
Think About It
Why should farmers consider the salinity of the soil in which they grow crops?
Answer: Farmers need to consider the salinity of soil, because the movement of water into and out of plant cells depends on the solute concentration of their environment. In soil high in saline, water will be drawn out of root cells causing the cells to shrivel, and the plant to die.
Teacher Support
- Student demonstrations should include the transport of different molecules across plasma membranes, which is shown in the illustration. Molecules of oxygen, carbon dioxide, and water have no charge and so pass through membranes by simple diffusion. Na+ and K+ have a charge and require a transport protein (the sodium–potassium pump) in order to move across the plasma membrane via active transport.
- The College Board® presents an expanded diffusion/osmosis activity on which this lab investigation is based. Please see Investigation 4 in the AP Biology Investigative Labs: An Inquiry-Based Approach.
Teacher Support
Observing Osmosis in Model Cells
Materials:
3 dialysis bags
3 medium-sized beakers
Stock starch solution
Distilled water
Iodine dropper bottle
Thread
Balance or scale
Preparation:
To prepare the percent starch solutions, determine the volume of the solution you wish to use (e.g., 100 ml) and add the mass of solute, in grams, equivalent to the desired percent concentration:
Percent solution = [Mass of solute (g) / Volume of solution (ml)] × 100 Label each dialysis bag with one of the three concentrations: 1%, 25%, and 60%. Then, moisten the dialysis tubing to make it easier to open. Fill each tube with the corresponding solution about three-quarters of the way fill in order to leave space to tie off the top of the bag. Tie the tops of the bags tightly with standard thread. Do not place the bags in the iodine solution yet, as they will first be weighed in front of the students.
With students present, explain to them the details of the setup and show them how iodine is used as an indicator for the presence of starch. Also explain that dialysis tubing is semipermeable, as it contains pores that permit the passage of small ions and molecules, like water; but will not permit the passage of larger molecules, like proteins. In this way, the dialysis bag models a semipermeable cell membrane.
- Fill each beaker about three-quarters of the way with distilled water. Add 3–8 drops of iodine, based on the strength of your iodine, and stir so that the solution is yellow in color.
- Weigh each dialysis bag and record the weights on a chart visible to the class.
- Ask students: If each dialysis bag were a cell, would it be hypertonic, hypotonic, or isotonic relative to the distilled water? How quickly will water flow into or out of the dialysis bag by osmosis? What will happen if the starch solution comes in contact with the iodine solution?
- Immerse the dialysis bags in the iodine solution for 45 min to 1 hour. While waiting, ask students to predict how osmosis will affect the weight of the three dialysis bags. Will the weight of the bags increase, decrease, or remain the same?
- Remove the bags and carefully rinse them under a gentle tap. Pat the bags dry to remove excess water. Weigh each bag and place the results on the board.
- Ask students: Was both water and starch able to pass through the dialysis tubing? How do you know, based on your results? Did the concentration of the starch solutions affect the rate of osmosis? Why would this occur?
Results:
Starch molecules cannot pass through the dialysis tubing. However, the iodine solution can pass from the beaker into the dialysis bag. This turns the starch solution from colorless to purple. The amount of iodine that diffuses into the bag is related to the concentration of each solution. As the starch concentration increases, more iodine solution will diffuse into the bag, causing the bag to increase in weight.
This lab is an application of Learning Objective 2.4 and Science Practices 1.4 and 3.1 and Learning Objective 2.5 and Science Practice 6.2 because as students collect and analyze data; in addition, as part of their experimental design, students draw conclusions from the data.